K. Saritha and Prof. N.V. Nanda Kumar
Abstract
Thevetia peruviana seed carboxyl esterase was employed as biosensor for determination of soluble selenium compounds by colorimetric-enzymatic method in fortified soil and water samples. This method was reported for the first time for selenium determination by employing a plant biosource. The method is based on the Thevetio peruviana seed carboxyl esterase inhibition versus selenium amounts. The minimum amount that can be determined is I pg for sodium selenite and selenium dioxide. The method might find application in bioassay of selenium employing a plant biosource. Appropriate clean up procedure was developed suitable for enzymatic method of determination of selenium compound.