SHILVIYA BHAT, RAJESH V. WAGH, NITIN MEHTA AND OM PRAKASH MALAV
Abstract
The present study was planned to optimize the extraction protocol to obtain the antioxidant-rich bioactive extracts from Spirulina platensis (SPL) and Garcinia indica (GID). Different solvents, viz. ethanol, methanol, and water, were used to finalize the best extracting solvent. Response surface methodology (Box- Behnken design) was used to estimate the optimum extraction conditions for each parameter with three different variables, viz. extraction temperature ranging from 60-80 oC, extraction time from 5-20 min, and solvent concentration 55-65% ethanol for both the Spirulina platensis and Garcinia indica extracts. Extractâs antioxidant efficiency was recorded by evaluating three responses, viz. ABTS 2,2?-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), DPPH (1,1-diphenyl-2-picrylhydrazyl) and TP (Total Phenolic content). Optimized results indicated that the highest antioxidant efficacies were obtained at the concentration of 60% with an extraction time of 15 min for SPL and 10 min for GID; extracting temperature of 65 oC for SPL and 75 oC for GID extract. Under these optimization processing conditions higher antioxidant activity of GID extract, i.e., DPPH-69%, ABTS-50.5%, and TP-323.93 mg/100g DW, than the SPL extract, DPPH-54.4%, ABTS- 36.3% and TP-260.1 mg/100 g DW. It can be concluded that RSM can be effectively used for optimizing the processing protocols and conditions of extraction to get bioactive components from natural plant materials.