S.H. BASAVARAJAPPA, C. LATHA AND N.S. RAJU
Abstract
Soil samples obtained from the lake bed were used to screen heterotrophic nitrifying bacteria as an alternate source of biological removal of nitrogen compounds in contaminated lake water. Primary and secondary enrichment were carried out on the bacterial isolate. Confirmatory tests were performed on culture samples that showed nitrate production. The presence of nitrifying bacteria was demonstrated by a change in color from pink to yellow and a drop in pH. Bacillus sp. is the genus from which the ammonia-oxidizing bacteria are isolated and identified using. 16SrRNA Bacillus pumilus, isolated from the beds of Kukkarahalli lake soil, was found to be a bacterial resource for nitrification and to have a potential bioremediation function in deoxidized nitrogen-contaminated water in this research. The ability of Bacillus pumilus to adsorb nitrogen compounds from eutrophicated water bodies was tested in a batch environment. Bacillus pumilus was successfully used in the laboratory to remove ammonia, nitrite, and nitrate nitrogen from synthetic solutions.