Renu Sharma and Deepak Mishra
Abstract
Pleurotus florida, commonly referred to as oyster mushroom, is a significant member of the Pleurotaceae family. It ranks as the second most widely cultivated mushroom variety in India. Oyster mushrooms are recognized as a wholesome food suitable for all age groups, boasting numerous medicinal properties. The current study aims to assess its molecular profiling and phylogenetic evolution studies by using RAPD genetic markers. In this a set on 75 RAPD markers test among 7 different samples of oyster mushroom collected from different localities of Vindhya region, out of which only 15 RAPD produces reproducible band patterns. After PCR amplification followed by gel documentation band scoring was for determination of the polymorphism percentage, pic value, Jaccard similarity coefficient. In the phylogenetic evaluation studies, pair wise genetic similarities with regard to RAPD marker ranged from 0.54 to 0.80. UPGMA dendrogram were generated based on Jaccardâs coefficient. Oyster, species were grouped into two clusters. First cluster contained four species P1, P2, P3 and P4 whereas, second cluster consist P5, P6 and P7 showed maximum genetic similarity with each other and grouped together.