Ankita Singh, A.K. Singh, Kadambari Tiwari, Ravindra Kumar, Yuvraj Singh Maurya, Vimal Kumar Yadav and Aman Singh
Abstract
The investigation titled âEffect of Putrescine and Spermidine through Seed Priming to Mitigate the Effect of Salinity in Rice (Oryza sativa L.)â was conducted during the Kharif season of 2024â25 at the Crop Physiology Farm, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya (U.P.). The experiment followed a Completely Randomized Block Design with three replications, seven treatments, and two rice varieties viz., salt-susceptible-IR 64 and salt-tolerant-CSR 36. Treatments included a control (distilled water) and seed priming with spermidine and putrescine at concentrations of 0.01 mM, 0.02 mM, and 0.03 mM. Observations on growth, biochemical parameters, yield, and yield attributes were recorded at 30, 60, and 90 days after sowing (DAS). Salinity stress significantly reduced plant height, tiller number, and dry matter accumulation, but seed priming with polyamines effectively improved these parameters. Among all treatments, spermidine at 0.01 Mm (T1 ) showed the most positive impact on growth, biochemical traits (such as chlorophyll, carotenoids, Total Soluble Sugars (TSS), and proline), antioxidant enzyme activity (CAT and SOD), and yield, followed by putrescine at 0.02 mM (T6 ). While the salt-tolerant variety, V2-CSR 36 showed better performance under salinity than V1-IR 64, the seed priming effects were more pronounced in IR 64. Overall, seed priming with spermidine and putrescine was beneficial for enhancing salinity tolerance and yield in both varieties. The study concludes that seed priming with polyamines could serve as a promising strategy for improving salinity stress tolerance in rice. Although, further research is required to refine the dosage and timing of application for different crops.