Ecology, Environment and Conservation Paper


Vol.31, August Suppl Issue, 2025

Page Number: S89-S94

IN-VITRO EVALUATION OF DOSE-DEPENDENT CYTOTOXICITY OF ZINC OXIDE NANOPARTICLES SYNTHESIZED FROM CARALLUMA FIMBRIATA (WALL) ON A549 LUNG CARCINOMA CELLS VIA MTT ASSAY

Sharad D. Dandekar and Jalindarnath G. Bagal

Abstract

Lung cancer, particularly non-small cell lung cancer (NSCLC), presents significant challenges in terms of treatment and prognosis, with conventional therapies often showing limited long-term effectiveness. This study explores the potential of zinc oxide nanoparticles synthesized from Caralluma fimbriata (Wall) (CF1Np) as a novel therapeutic agent against A549 human lung carcinoma cells. Employing eco-friendly green synthesis methods, the study highlights both the biocompatibility and sustainable aspects of nanoparticles production. Characterization of CF1Np demonstrated a size range of 14-21 nm, as confirmed by techniques including X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), and Scanning Electron Microscopy (SEM). The cytotoxic effects of CF1Np were assessed using the MTT assay, which revealed a dose-dependent reduction in cell viability, with an IC of 118.57 ?g/ml, indicating lower potency compared to the standard chemotherapeutic agent 5-Fluorouracil (IC of 23.37 ?g/ml). Although CF1Np exhibited some anticancer activity, its effectiveness suggests a need for further optimization to enhance its therapeutic potential. This research emphasizes the importance of plant-derived nanoparticles in cancer treatment and underscores the necessity for ongoing exploration of biosynthetic approaches in developing effective anticancer therapies.