Rishi Srivastava, Rajesh Sharma, Shweta Sonam and Shree P. Tiwari
Abstract
Natural products obtained from microorganisms are major sources of antibiotics but discovery of new antibiotics is an inextricable challenge. The resistance-guided approach of isolation exploits the self-protection mechanism of antibiotic producers to isolate the microorganisms producing antimicrobial compounds of selected antibiotic scaffolds. The frequency of new antibiotic discovery from the conventional sources has been decreased significantly. Gomti river is still unexplored regarding isolation of antibiotic producing bacterial strains. In the current study, antibiotic-producing bacteria isolated from the Gomti river by using resistance-guided approach were assessed for their potential to produce antibiotics. The study revealed that the turbidity equivalent to 3.0 McFarland standard was suitable for extraction of antibiotics. The production of antibiotics was assessed on day-to-day basis based on the zone of inhibition formed. Isolates RS1, RS2, RS3, RS6 and RS7 were exhibited significant potential to produce antibiotics against strains of Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli and Enterobacter sp.