Jayashri P. Nanaware and Pathade G.R.
Abstract
Molasses-based distilleries produce effluent that has a high oxygen demand and is highly coloured. Microbial decolorization is an economical and ecologically sustainable method for treating this wastewater. In the present study a total four number of promising Yeast obtained from total 40 isolates and they were designated as IY1, IY2, IY3 and IY4. All the four yeast isolates were studied for their ability to decolorize distillery spent wash qualitatively and quantitatively along with removal of organics. The isolate IY2 showed considerable quantitative decolorization at different concentration of spent wash (10%, 20% and 40%). The IY2 showed highest degree of distillery spent wash decolourization (79.36%) at 10% concentration of spent wash. This isolate was identified as Pichia kudriavzevii by 18SrRNA gene sequencing with NCBI accession number of PQ396170 and phylogenic tree is also generated. This yeast isolate is obtained from soil in the region of distillery lagoons Rethare region, Maharashtra which has promising potential to decolorize melanoid in pigment of distillery effluent. The 92.62% COD and 83.39% BOD removal was also obtained also with decolorization at 30 0C after 10 days incubation. This study may be used as a strategy to reduce environmental contamination and human health risks near the distillery.