Aarti Meena and Shiny Sharma
Abstract
The present investigation was carried out to estimates effect of ultrasonication on cell biovolume, biomass and carbohydrate concentration in Chlorella culture at laboratory of Synthetic Ecology and Environment Biotechnology, Department of Environmental Science, School of Earth Sciences, Central University of Rajasthan, Bandarsindri, Ajmer. To extract valuable compounds from algal biomass, various cell disruption techniques are used, including ultrasonication, which is a green and novel method that requires less solvent and energy consumption. Ultrasonication produces vibrations and cavitation effects that disrupt the micro algal cell wall and release intercellular products into the surrounding medium. The study aims to investigate the effect of ultrasonic treatment of different durations on cell biovolume, biomass, and biomass productivity, which was given to the Chlorella sp. culture. The biomass and biomass productivity concentration increased from 29.9 ± 0.00240 mg/l (control) to 66.8 ± 0.01485 mg/l, 1.275 ± 0.5033 (control) to 3.71 ± 0.4163 mg/l/day respectively. An optimized duration of Ultrasonication stress was applied which result in the increase in carbohydrate concentration in stationary phase which was 34 ± 0.1732 mg/l compared to 21 ± 0.1527 mg/l in the control.