Preeti, Manishi Mukesh, Pamela Singh, Shivam Kumar Dubey, Gaurav Bhakri, Anurag Kumar, Vipul Chaudhary, Ranjit S. Kataria, Saket Kumar Niranjan, Ashok Kumar Mohanty and Monika Sodhi
Abstract
Cowâs milk is an essential part of the human diet as it provides vital nutrients and exhibits medicinal properties. It is rich in high-quality proteins, fats, sugars, vitamins, and minerals, with over 95% of its proteins consisting of caseins and whey proteins. Among these, ?-casein exists in several genetic variants, categorized into A1 and A2 types. During digestion, A1 milk releases a peptide called ?-casomorphin-7 (BCM-7), which has been linked to adverse health effects, including cardiovascular and neurological disorders. In contrast, A2 milk does not release BCM-7 and is considered a safer alternative. The aim of this study is to find out the effects of A1 and A2 milk on biochemical parameters in C57BL/6J mice fed a highfat diet (HFD). A total of 25 male mice were divided into five groups: control (standard chow), HFD, HFD + A1A1 milk, HFD + A2A2 milk, and HFD + A1A2 milk. The results showed that the HFD group had elevated levels of blood glucose, ALT, AST, triglycerides, and uric acid compared to the control group. While no significant differences were observed between the A1A1 and A1A2 groups. The A2A2 milk group exhibited significantly lower blood glucose, ALT and urea compared to the A1A1 and HFD groups. Other markers, such as albumin, total protein, and creatinine, did not significantly varied amongst the groups. Overall, the study indicates that A2 milk may offer health benefits in reducing liver enzymes and triglycerides in the context of a high-fat diet.