Ecology, Environment and Conservation Paper


Vol.31, Feb Suppl Issue, 2025

Page Number: S45-S50

BIOCHEMICAL CHARACTERIZATION OF XANTHOMONAS CAMPESTRIS SPV. VESICATORIA IN CHILLI PEPPER (CAPSICUM CHINENSE)

Laiphrakpam Pinky Chanu, Ajit Kumar Ng, Lakshmikanta Khundrakpham, Bijayalakshmi Devi Nongmaithem, Rocky Thokchom, Lenin Laishram, Lorinda Kangabam, Precious Irom and Harvinder Kaur Sidhu

Abstract

Solanaceous crops hold a significant importance in the Indian agriculture sector, widely consumed in India but it is vulnerable to the infestations of Xanthomonas campestris pv. vesicatoria (Xcv). This study aimed at identifying and analyzing the biochemical properties and profiles of the bacterium Xanthomonas campestris pv. vesicatoria specifically in relation to its interaction with chilli pepper plants. Chilli samples were collected from different parts of Manipur, India. In total nine isolates were cultured in two different media- Sucrose Peptone Agar (SPA)/ Hawyard’s Medium and Yeast Dextrose Calcium carbonate (YDC), and underwent the biochemical test viz., starch hydrolysis, KOH test, catalase test, motility, indole test, etc. During the investigation, nine isolates were taken and undergo various biochemical tests. Six isolates produced positive reaction to KOH solubility test, gram negative. All the isolates were motile and showed negativity to indole test. The morphological and biochemical investigation indicates that the four isolates viz., A3I, A3II, B3I and B3II belongs to the species Xanthomonas campestris pv. vesicatoria, which is known to cause the bacterial leaf spot disease of chilli pepper.