Ecology, Environment and Conservation Paper


Vol.30, October, Suppl. Issue, 2024

Page Number: S460-S466

TO DETERMINE THE ANTIOXIDANT MECHANISM OF ACTION, BIOCHEMICAL COMPOUNDS IN TECOMA STANS (L. JUSS. EX KUNTH) WERE ANALYZED USING GC-MS, HPLC, UV-VIS, AND FTIR TECHNIQUES

T. Durgadevi, K. Devika, G. Anburaj and C. Thillaiyadi Valliammai

Abstract

The alkaloids found in Tecoma stans (L.Juss.ex Kunth) are well-known and have been utilized extensively in traditional Chinese medicine. On the other hand, the phenolic compounds have not been sufficiently studied. Thus, the objective of this study was to qualitatively and quantitatively examine the phenolic chemicals found in the plant’s roots and leaves. Either methanol or ethanol were used in the extraction processes; however, ethyl acetate and water worked better than methanol and ethanol. Phenolic chemicals were more abundant in the leaves than in the roots. The DPPH technique yielded values for the antioxidant activity of 46.59 g/ml, 40.51 g/ml, and 34.89 g/ml, in that order. The existence of phenolic compounds was confirmed by FTIR and the UV-VIS spectra. Using negative and positive ionization, GC-MS was used to examine the phenolic profile. A total of 15 compounds were identified, and HPLC analysis revealed the existence of 8 compounds that would be useful in pharmaceutical and medical applications.