Ecology, Environment and Conservation Paper


Vol.30, October, Suppl. Issue, 2024

Page Number: S103-S107

ANTIBACTERIAL, ANTIOXIDANT, AND CYTOTOXICITY ANALYSIS OF GREEN SYNTHESIS SILVER NANOPARTICLES FROM OREGANO, ROSEMARY, AND THYME LEAF EXTRACT

Namrata Budhiraja, Sreenivasulu Peta and Devendra Singh

Abstract

Despite being still in its early phases of development, nanotechnology has grown in popularity over the last few years. This is due to its capacity to transform metals into nano-sized particles, altering their chemical, physical, and visual properties. Nanotechnology is important in a variety of scientific and technological domains because of its unique properties. In this study, silver nanoparticles were successfully synthesized by using oregano, rosemary, and thyme leaf extract through a sustainable and eco-conscious approach. The nanoparticle thus produced has been characterized by using TEM. Further, the antibacterial activity of the synthesized nanoparticles was also assessed by using a disc diffusion assay. Finally, the antioxidant and cytotoxicity were also analyzed. The results indicate the successful synthesis of NPs, which showed potent antibacterial properties. The maximum zone of inhibition against E.coli was shown by the nanoparticles synthesized with oregano extract (16.66 mm). Additionally, the cytotoxicity assay conducted on the SW480 cell line confirmed that the nanoparticles are either non-toxic or minimally toxic. However, a dose-dependent cytotoxicity is also seen in vitro. Although green synthesis is gaining popularity due to its eco-friendly approach, further clinical analysis needs to be done.