Ecology, Environment and Conservation Paper


Vol.30, Issue 3, 2024

Page Number: 1143-1148

EFFICIENT MICROPROPAGATION TECHNIQUES FOR FINGER LIME CULTIVATION WITH EMPHASIS ON SHOOT TIP EXPLANTS

Supreetha B.G., Prakasha D.P., Kulapati Hipparagi, Prabhulinga G., Gurumurthy S.B., Ratnakar Manjunath Shet and Ashok

Abstract

Finger lime (Citrus australasica F. Muell) is a rare citrus fruit from Australia that is highly valued in the world’s citrus industry. It has vesicles that resemble caviar. The goal of this work was to develop a productive in vitro micropropagation technique for Finger lime, providing a long-term way to produce large quantities of this important crop for the economy. Our study aimed to maximize the usage of plant growth regulators for improved induction and initiation of shoots. Tests were conducted using combinations of cytokinins (Benzyladenine and Kinetin) and gibberellic acid (GA). Results showed that a combination of BA (0.25 mg/l) and Kinetin (0.25 mg/l) was the most effective for shoot initiation from explants. Further, adding GA (1 mg/l) improved shoot initiation and elongation. The best response was achieved when explants were exposed to combination of BA (0.25 mg/l), Kinetin (0.25 mg/l) and GA (1 mg/l). This protocol significantly improved shoot initiation and growth, providing an efficient Finger lime micropropagation method. This micropropagation protocol promises sustainable Finger lime cultivation and commercial production. It ensures a stable supply of planting material and promotes genetic uniformity among propagated plants. The research paves the way for improving Finger lime cultivation techniques and disease-free planting material development, boosting the productivity and economic viability of Finger lime production.