Suraj Jadhav, Suresh Jagtap, Anand Zanwar and E. A. Singh
Abstract
The ââ¬ËSarivaââ¬â¢, a drug having diverse uses in Ayurveda. Under the name ââ¬ËSarivaââ¬â¢ four different species have been mentioned and supplied viz. Cryptolepis buchananii Roem & Schult., Decalepis hamiltonii Wight. & Arn., Hemidesmus indicus (L.) R.Br., and Ichnocarpus frutescence R. Br. Vanillin is a key active constituent of these plants. Considering the availability of plant material in the market there is an ambiguity in supplying the authentic species as ââ¬ËSarivaââ¬â¢. This study was planned to study the effective species as ââ¬ËSarivaââ¬â¢ using biological activity as well as content of Vanillin using HPTLC fingerprints, and antioxidant abilities. A precise method was developed for detecting vanillin concentrations in various ââ¬ËSarivaââ¬â¢ extracts, using suitable mobile phase. The method was suitable for routine examination of vanillin in ââ¬ËSarivaââ¬â¢ spp. providing reliable and consistent results. The highest peak area for vanillin was reported in Hemidesmus indicus, followed by Decalepis hamiltonii, Ichnocarpus frutescence, and Cryptolepis buchananii based on area under curve, indicating content variation in tested samples. The study also assessed the antioxidant capability of different ââ¬ËSarivaââ¬â¢ drugs using DPPH, FRAP, and ABTS assays. Hemidesmus indicus showed significantly higher antioxidant activity followed by Decalepis hamiltonii, Cryptolepis buchananii and Ichnocarpus frutescence. These findings suggest that Hemidesmus indicus and Decalepis hamiltonii have similar natural antioxidants with potential health benefits. Considering the less availability of Hemidesmus indicus, Decalepis hamiltonii can be potent substitute as ââ¬ËSarivaââ¬â¢. Further research is needed to explore the specific mechanisms of action and potential applications of Vanillin in preventing oxidative stress-related diseases.