Anupa, T., Somkuwar, R.G and A.K. Sharma and Alok Gupta
Abstract
The current study, titled ââ¬ÅStudies on in vitro Surface sterilization and disinfection procedure for in vitro propagation of grape rootstocks was conducted at the Tissue Culture Laboratory, ICAR-National Research Centre for Grapes Pune, from 2018-19 and 2019-2020. maximum explant establishment (82.3 %) and (80.3 %) were recorded in Dogridge and 110 R, respectively. When compared to May, June and July, the month of ââ¬ËAprilââ¬â¢ is ideal for culturing nodal cuttings. Both rootstocks had a similar pattern in terms of the monthto- month establishment. Carbendazim @ 1 g/l for 2 hours was shown to be the most effective in reducing contamination and promoting plant establishment in Dogridge (11.6 %) and 110R (13.3) rootstocks respectively. In Dogridge and 110 R rootstocks, charcoal @ 1g/l was found to be effective in reducing browning (12.3 and 12.6 %) and contamination rate (11.3 and 11.0 %). In Dogridge and 110 R. Surface sterilization with mercuric chloride for 7-10 minutes was shown to be effective in reducing contamination rate for nodal explants in Dogridge (19.6 %) and 110 R (22.6 %).