Altamemy and A.K. Akool
Abstract
Thirty samples of Greylag goose- Anser anser were collected from Al-Dalmage marshes in Wasit province ââ¬â Iraq. The specimens of tissue was collected from brain and liver and grinded by grinder after treated with liquid nitrogen to prepare ââ¬Ëtissue powderââ¬â¢, Genomic DNA purification kit (promega. USA) was used to extraction of sampleââ¬â¢s DNA according to company instructions and tested by Real time PCR using primers and probe targeting B1 gene of Toxoplasma gondii parasites. There are nine out of 30 (30%) of duckââ¬â¢s brain samples was positive. Whereas 14 out of 30 (46.6%) samples of duckââ¬â¢s liver was positive.