VALORIZATION OF YEASTS POTENTIAL ASSOCIATED TO MOROCCAN PICHOLINE OLIVE VARIETY IN THE TREATMENT OF OLIVE MILL WASTEWATERY. BOUDALLAA, A. EL ANTARI AND KH. BOUKACHABINE
The aim of this study is yeast screening from olive tree habitats (soil, leaves, olives, olive oil, pomace and OMW) with a potential application in degrading olive mill wastewater (OMW) from three processes: three-phase continuous process, semi-modern (hydraulic press) and traditional systems, from Marrakech Safi region (Morocco). Thus, enzymes potential production from the interesting isolates were characterized. On 185 yeasts previously isolated from different habitats, 65 isolates were selected with a potential growth on solid OMW based medium and belonging to 5 genera: Candida, Cryptococcus, Pichia, Debaryomyces and Schwanniomyces. From these genera, the species, which gave a considerable growth magnitude, were kept for detoxification essay of OMW from continuous process. The results of detoxification showed that the species Cryptococcus albidus, Debaryomyces hansenii var. fabrii and Schwanniomyces occidentalis var. occidentalis present the best percentage of reduction of BOD5 and polyphenol (50% removal reduction). From the 65 isolates, only 8 isolates species, Cryptococcus albidus, Candida gropengiesseri, Cryptococcus albidus, Debaryomyces hansenii var. fabrii, Schwanniomyces occidentalis var occidentalis, Candida magnolia, Candida boidinii and Pichia norvergensis showed a strong growth on liquid OMW. Variable activity profiles are shown in screening of lipase, amylase, Î²-glucosidase and catalase, ranging from absence to high activity. The results showed that 2 yeasts Cryptococcus albidus isolated from olive and Debaryomyces hansenii var. fabrii from OMW display a high lipase and amylase activity belonging to the genera, when almost all strains display moderate or strong Î²-glucosidase and catalase activity.
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