Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 21, Issue 3, 2019; Page No.(648-655)

ISOLATION, IDENTIFICATION AND CHARACTERISATION OF PLANT GROWTH PROMOTING BACTERIAL ENDOPHYTES FROM EMILIA SONCHIFOLIA (LINN.) DC

SITHARA K. URUMBIL AND ANILKUMAR M.

Abstract

Emilia sonchifolia (L.) collected from all the fourteen districts of Kerala, India, served as the source material for the isolation of endophytes. Based on visible morphological characters 65 different bacterial colonies were isolated which on further biochemical tests have reduced the number of isolates to six such as Bacillus subtilis (ES-1), Paenibacillus sp.(ES-2), Microbacterium sp.(ES-3), Bacillus cereus (ES-4), Bacillus aryabhattai (ES-5) and Micrococcus sp. (ES-6) based on 16S rDNA sequencing. Phylogenetic study of the isolates was done using MEGA7 to assess the evolutionary relationship. These isolates were screened for plant growth promotion activity by measuring the production of IAA, siderophore, ACC deaminase and solubilisation of phosphate. Among these isolates Paenibacillus sp. showed highest tolerance to salinity (8%NaCl) while Micrococcus sp. was the most sensitive. Temperature tolerance of the isolates revealed that they were capable of growing both in low as well as high temperatures (-1°C to 60 °C). Further analyses of the isolates are in progress for the detailed molecular characterisation.

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