ANALYSIS OF TISSUE RESPONSE AFTER SUBCUTANEOUS IMPLANTATION OF DEMINERALIZED FREEZE-DRIED BOVINE CORTICAL BONE MEMBRANEDANANG PRIYO UTOMO, DAVID B. KAMADJAJA, FIKA RAH AYU, PRATIWI SOESILOWATI, ACHMAD HARIJADI AND R. SOESANTO
Guided bone regeneration (GBR) for alveolar bone augmentation commonly uses collagen membrane made from bovine pericardium membrane (BPCM). However, it has been associated with prolonged biodegradation. Due to that reason, an innovation is needed to manufacture an alternative of GBR membrane which is demineralized freeze-dried bovine cortical bone membrane (DFDBCBM). However, its biocompatibility needs to be revealed by evaluating tissue response after DFDBCBM implantation. This study aims to analyze tissue response to DFDBCBM after subcutaneous implanted in rats dorsum compared with that of BPCM. This study used 32 samples of rat divided into 2 groups (DFDBCBM and BPCM). Samples from each group were sacrificed after 7, 14, 21, and 28 days of subcutaneous implantation. The specimens were processed and stained with Hematoxylin-Eosin for histology examination to evaluate the thickness of the fibroblast capsule and the quality of the fibrous capsule as well as interface tissue. Data collected was statistically analyzed with p value of < 0.05. This study showed no statistically difference in the quantity of fibroblast capsule and quality of fibrous capsule as well as interface tissue between DFDBCBM group and BPCM group (p> 0.05). Demineralized freeze-dried bovine cortical bone membrane elicits similar tissue response compared to bovine pericardium membrane. The result indicates that DFDBCBM is a potential alternative for guided bone regeneration membrane.
Enter your contact information below to receive full paper.