Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 18, Issue 3, 2016; Page No.(731-734)

A STUDY ON EFFECTS OF INOCULUM MEDIUM AND FED BATCH CULTIVATION FOR POLYHYDROXYALKANOTES SYNTHESIS BY PSEUDOMONAS PUTIDA MTCC 102 TYPE B

NATARAJAN AHILANANTH AND SUBRAMANIAN RAMALINGAM

Abstract

The present study portrays the synthesis of mcl PHAs from Pseudomonas putida using glucose as the sole carbon source. In spite of the fact that glucose was revealed to be efficient for mcl PHA production, unlike fatty acids it obliges nitrogen restriction for proficient PHA accumulation. In this study, we have demonstrated the impact of inoculum medium in batch cultivation and also developed the exponential feeding strategy to increase the PHA concentration in fed batch cultivation .There is a substantial increase in PHA concentration in fed batch cultivation hen compared with the batch cultivation. The final biomass and PHA concentrations achieved were 2.8 g/L and 0.62 g/L respectively.

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