Ecology, Environment and Conservation Paper

Vol 22, Issue 2, 2016; Page No.(755-761)

ALLELIC DIVERSITY OF SSR MARKER ANALYSIS AND CLUSTERING TO FIND THE VARIABILITY AMONG THE PARENTS TO PRODUCE THREE LINE HYBRIDS IN RICE (ORYZA SATIVA L.) UNDER AEROBIC CONDITION

R. Sathya

Abstract

A parental survey was conducted subjecting 21 parents using SSR markers. The survey indicated that out of 26 SSR markers, seven markers were found to be highly polymorphic. Based on the PIC values, the polymorphism observed in seven SSR markers (RM206, RM278, RM324, RM279, RM303, RM334 and RM281) among the rice cultivars in the present study demonstrated the effectiveness of this method in determining genetic variation. The PIC values for these seven markers were > 0.50. Genetic similarity values among the 21 rice genotypes used led to the construction of dendrogram. At 73 per cent level of similarity, UPGMA cluster diagram showed VIII groups with additional clusters within each group. The clustering pattern revealed that the genotypes within each cluster had superior performances for one or more traits. The genotypes within each cluster may have more similarity and hence cannot be used in the improvement of traits whereas more diversity occurs between clusters. Hence, the genotypes of one cluster may be crossed with the genotypes of another cluster. The genotypes IR 79128A, IR79156A, IR 7925A-428-2-1-1R, IR 69726- 29-1-2-2R, IR 79582-21-2-2-1R, IR 80402-88-3-1-3R, KMP-149, KMP-148, BR-2655 and BI-33 belonging to any one of the VIII clusters exhibited superior performances for different traits. These parents are highly suitable for introgression of important traits which contribute yield improvement in aerobic rice breeding.

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