Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 18, Issue 1, 2016; Page No.(239-247)

RAPID IDENTIFICATION OF GENETIC VARIATION OF ASPERGILLUS FLAVUS ISOLATED FROM DIFFERENT WATER SOURCES BY RANDOM POLYMORPHIC DNA ASSAY

BANU NARASIMHAN AND PAVITHRA PUSHKAR

Abstract

Aspergillus flavus is the second most important Aspergillus species causing human infections. The importance of this fungus increases in regions with a dry and hot climate. In addition, many A. flavus isolates produce aflatoxin B1, the most toxic and potent hepatocarcinogenic natural compound ever characterized. The use of RAPD (Random – Amplified Polymorphic DNA) method to determine variability has the advantages of being easy to perform, versatile regarding primers, low-cost and, especially, not requiring previous knowledge on the genetics of the microorganism under study. The objective of the present study was to compare the genomic profile of different strains of A. flavus isolated from different kinds of drinking water collected from different places of Tamil Nadu, through RAPD analysis. In the present study, two distinct major profiles were observed for Aspergillus flavus by RAPD analysis. RAPD profiles seemed to be considerably less variable within the various isolates of Aspergillus flavus. Among the five isolates, 1, 3 and 5 were more closely related than 2 and 4.

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