MOLECULAR IDENTIFICATION OF ESCHERICHIA COLI BETA – LACTAMASE TEM AND SHV BY PCR IN TEHRAN HOSPITAL WASTE WATERTAHEREH NESHATI, FATEMEH NOORBAKHSH AND SHOHREHZARE KARIZI
Antibiotics are one of the most valuable medicinal substances entering the hospital waste water cause as problems for environment. Over use of antibiotics and their entry in to the hospital waste water lead to increasing of antibiotics resistance bacteria (1). TEM and SHV beta-lactamase enzymes are the most important factor in resistance to beta- lactamaseantibiotic between gram negative bacteria. Evidence is increasing of presence of B-lactamase enzymes in hospital effluents. The purpose of this study was to investigate the presence of antibiotic resistance genes in the field of blashv and bla TEM is in hospital waste water. Fifty two strains of Escherichia coli were isolated from hospital waste water and antibiotic resistance is determined by disk diffusion method. Antibiotics studied were Cefotaxime, Ceftazidime, Ceftizoxime, Imipeneme, Sterptomycin, Ciprofloxacin, Amoxicilin, Gentamicin. DNA extracted from isolated bacteria and finaly PCR was performed for the detection of blaTEM and bla SHV gene. Antibiotic susceptibility test revealed that 35 strains of 52 isolates were resistant to amoxicillin. PCR amplification revealed bla TEM and bla SHV gene respectively 33 (% 63.1) and 31(%59.5) of E.coli isolated of waste water. Phenotypic test results showed that the production of beta-lactamase enzymes of the strains studied is high. And finally PCR studied showed a high frequency of of TEM and SHV.
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