Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 18, Issue 1, 2016; Page No.(101-106)

LIPID CONTENT FROM MONOCULTURE OF MICROALGAE CHLORELLA ZOFINGIENSIS DÖNZ AND MIXED CULTURE OF GLAGAH ISOLATE IN LABORATORY SCALE AND RACEWAY POND FOR BIODIESEL PRODUCTION

EKO AGUS SUYONO, MATIN NUHAMUNADA, NASTITI RAMADHANI AND RAMDHANIYAH

Abstract

Mixed cultures of Glagah isolate is potentially used for enhancing the content and productivity of lipid in the biomass produced from large scale cultivation of microalgae. Therefore, it can be used to replace monoculture systems for the production of biodiesel. Thus, this study was conducted to determine the content and productivity of lipid in the biomass produced from Glagah isolate as model of mixed culture system and Chlorella zofingiensis as a model of monoculture systems, both on laboratory scale and raceway pond. This study used Chlorella zofingiensis Dönz and mixed culture from Glagah isolate. The algae was cultivated for 7 days on laboratory scale using the Bold’s Basal Medium and on raceway pond with modified Walne’s medium and BG11. Growth was measured by counting the cell density. Neutral lipid content was measured using Nile Red staining method. Dry weight was calculated every 2 days to get lipid productivity. It can be concluded that the highest lipid percentage was shown by isolates Glagah in raceway pond, which was about 2.29%. The highest lipid content was achieved by Glagah isolates were cultivated in raceway pond, reaching 4.5 mg L-1. The cell lipid quota Glagah isolates cultivated in raceway pond was higher than other cultures, reaching 2.64 ng-1 cells. In raceway pond, isolates Glagah had higher productivity accounted for 0.71 mg L-1 day-1.

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