Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 17, Issue 4, 2015; Page No.(897-899)

EFFECT OF ARGAN OIL-HYDROGEN PEROXIDE MIXTURE ON MYCOBACTERIUM TUBERCULOSIS IN VITRO

ANWAR K. AL-SAFFAR AND HUSSEIN O.M. AL-DAHMOSHI

Abstract

– Objective: Tuberculosis (TB) is the threatful infectious disease that dwell about one third of world. Among middle east, Iraq occupy the fifth order country endemic with TB. Six mixtures of Argan oil: 1.5% H2O2 were prepared at different ratio: 1=0.75:9.25, 2=1:9, 3=1.5:8.5, 4=2:8, 5=2.5:7.5, 6=3:7 and mixed with Lowenstein-Jensen (L-J) medium. Seventeen Mycobacterium tuberculosis isolates got from the consultants clinic of respiratory and thoracic diseases in Hilla city-Iraq. All isolates were cultured on LJ medium containing the mixture of Argan oil:1.5% H2O2 in different ratio and control tube (LJ without mixture) also used. The growth was monitored per day and recorded at different intervals and the inhibitions (no growth) were recorded. The effect of Argan oil: 1.5%H2O2 mixture on the Mycobacterium tuberculosis were investigated over three incubation intervals (14th-20th, 21st-28th and after 29th day). The results revealed, the high Argan concentration mixture 2.5:7.5 and 3:7 gives excellent inhibitory effect on Mycobacterium tuberculosis during full periods of incubation with inhibition percentage (64.71%-82.35%). The current study aims to investigate the effects of argan oil on Mycobacterium tuberculosis and concludes the anti-mycobacterial activity of argan oil as a safe medication for prophylaxis and treatment of TB.

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