Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 17, Issue 3, 2015; Page No.(511-513)

CONFOCAL MICROSCOPY OF WOLBACHIA SPIN DROSOPHILA SIMULANS (INFECTED AND UNINFECTED WITH THE REVERSIDE STRAIN OF WOLBACHIA), ALSO D. MELANOGASTER INFECTED WITH THE POPCORN-STRAIN OF WOLBACHIA

ENDANG SRIMURNI KUSMINTARSIH

Abstract

Wolbachia isidentified using several different methods, such as light microscopy, electorn microscopy, and PCR. Recently, confocal microscopy techniqueis also developed to identify Wolbachia. Confocal microscopy is used to examine Wolbachia in Drosophila simulans infected with the Reverside strain of Wolbachia and D. Simulans uninfected after treated with tetracycline (DSRT), also D. melanogaster infected cysts in the testes of D. simulans infected with the Riverside strain of Wolbachia. Mean while, Wolbachia are absent from D. simulans treated with tetracycline. Subsequency, the sperm cycts in testes of D. melanogaster infected with the popcorn-strain of Wolbachia does not show Wolbachia either. Confocal microscopy technique is promosing for supporting rencent method in order to determine Wolbachia.

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