Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 17, Issue 1, 2015; Page No.(167-175)

ANTI-BACTERIAL ACTIVITY OF COW URINE EXTRACT OF NEEM AGAINST XANTHOMONAS ORYZAE THE CAUSATIVE AGENT OF BACTERIAL LEAF BLIGHT DISEASE OF PADDY

SUBBARAYAN SHANTHI, NALLUSAMY SIVAKUMAR, K. RAJAPANDIAN, ANNAMALAI PANNEERSELVAM AND NATARAJAN RADHA

Abstract

Bactericidal effects of different extracts of cow urine and neem (Azadirachta indica A. Juss) was studied against Xanthomonas oryzae pv oryzae, the causative agent of bacterial leaf blight disease of paddy. Fresh, incubated, condensed and chloroform extracts of cow urine were tested against X. oryzae pv oryzae. Similarly aqueous, hexane, chloroform, ethyl acetate, methanol, alcohol and cow urine extracts of A. indica were tested for their antixanthomonas activity. Cow urine extract of A. indica was more effective than the other extracts. Maximum antibacterial activity of cow urine extract of A. indica against X. oryzae pv oryzae was observed at 1600 μg concentration. Phytochemical analysis of incubated cow urine extract of A. indica showed the presence of flavonoids, quinines, alkaloids, coumarin, tannin, saponin and phenol. The GC-MS and FT-IR studies revealed the presence of oxiranes, dibutyl thalate, phenol, benzene dicarboxylic acid which could be the possible essential antimicrobial principles against X. oryzae.

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