HYDROLYTIC ENZYME PROFILING OF MODERATELY HALOPHILIC STAPHYLOCOCCUS XYLOSUS ISOLATED FROM SALINE SOILSH. J. BHOSALE, T. A. KADAM AND S. R. SUKALKAR
Extracellular hydrolytic enzymes derived from halophiles are an important group of biotechnologically relevant enzymes that find immense industrial applications. In the present study, 84 moderately halophilic bacteria were isolated from saline soil of Mumbai port, India and screened for their ability to produce hydrolytic enzymes including amylase, protease, lipase, cellulase, xylanase, pectinase, L-asparginase and inulinase using plate assay. 37 isolates showed amylase activity, 34 showed proteolytic activities whereas xylanase, cellulase, pectinase, lipase, inulinase and Lasparginase activities were observed in 25, 22, 7, 20, 2 and 17 isolates respectively. Hydrolytic activity scores of the isolates were determined depending on their ability to produce number of hydrolytic enzymes. Based on hydrolytic activity scores, the isolate MHB32 was selected as a potential hydrolase producer and identified as Staphylococcus xylosus after morphological and biochemical studies and by 16S rRNA analysis. The isolate showed maximum production of protease (77 U/mL) followed by amylase (56 U/mL), L-asparginase (43 U/mL), lipase (25 U/mL), xylanase (23 U/mL) and cellulase (17 U/ mL) respectively. The study revealed the significance of S. xylosus in having combined hydrolytic activities and may open up new applications in various fields of biotechnology.
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