Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 15, Issue 2, 2013; Page No.(263-272)

ISOLATION OF TDC GENE FROM ALOE VERA AND ITS CHARACTERIZATION AND CLONING

T. VASANTHA NAIK, H.S.RAVIKUMAR PATIL, B.R.VIJAY AVIN, H.A. SAYESWARA AND MAHESH ANAND GOUDAR

Abstract

The Aloe vera plant has been known and used for centuries for its health, beauty, medicinal and skin care properties. The name Aloe vera derives from the Arabic word “Alloeh” meaning “shining bitter substance,” while “vera” in Latin means “true.” 2000 years ago, the Greek scientists regarded Aloe vera as the universal panacea. Aloe vera contains number of potentially active constituents such as vitamins, enzymes, minerals, sugars, lignin, saponins, salicylic acids, amino acids and those which are mainly responsible for the properties of Aloe vera the primary and secondary metabolites. Tryptophan decarboxylase (TDC, EC 4.1.1.28) is the key enzyme in the early step of the TIA biosynthetic pathway and links primary metabolism to secondary metabolism by converting tryptophan into tryptamine, which is subsequently condensed with secologanin, resulting in strictosidine, the precursor to pharmaceutically important alkaloids such as vinblastine and vincristine. In the present study, the tdc gene isolated from Aloe vera. And after sequencing an 1160bp sequence of gene was got in the Aloe vera which was observed to be identical with tdc gene of C. roseus by BLAST analysis. Which inferred that the sequenced gene to be the gene coding tryptophan decarboxylase in Aloe vera. Hence the enzyme and the gene coding play an important role in biosynthesis of important secondary metabolites.

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