Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 15, Issue 2, 2013; Page No.(259-262)

ISOLATION IDENTIFICATION AND DETECTION OF BILE TOLERANCE OF LACTOBACILLI

HEMANT CHUNDAWAT AND HARSHADA JOSHI

Abstract

A total of 200 strains of lactobacilli were isolated on MRS agar medium from 24 samples comprising of 11 samples of cow milk, 8 samples of camel milk and 5 samples of curd. Among 200 strains 74 strains were recovered from cow milk samples, 82 from camel milk samples and 44 from curd samples. A total of 125 out of 200 isolates were appeared as rods, showed negative catalase reaction, yellow colonies on BCP supplemented MRS agar and also positive reaction for litmus milk. All the 125 isolates showed 194 bp amplified product there by confirming that they belong to genus Lactobacillus. Among 125 Lactobacillus isolates 45 Lactobacillus isolates showed bile tolerance as they were able to grow at minimum concentration of sodium taurocholate that was 0.2%. Among 45 Lactobacillus isolates 36 Lactobacillus isolates showed growth at fairly high concentration 0.3% of bile and out of them 22 Lactobacillus isolates showed growth up to maximum concentration (0.4%) of bile used in the study. These 22 Lactobacillus isolates can be used as potent probiotics as they have showed demonstrable tolerance to sodium taurocholate.

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