Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 11, Issue 4, 2009; Page No.(903-906 )

IN-VITRO REGENERATION OF SIEGESBECKIA ORIENTALIS L., A MEDICINAL PLANT

R. GEETHA AND G.V. GOPAL

Abstract

Multiple shoots were induced from nodal explants of Siegesbeckia orientalis L. on Murashige and skoog's basal medium supplemented with 1mg/L Benzyl Amino Purine within 4 weeks of culture.These shoots were sub cultured for in vitro rooting with MS medium supplemented with different auxins at various concentrations and the best results were obtained on MS+1mg/L Indole Butyric Acid. In vitro flowering was observed on a medium supplemented with 2 mg/L Benzyl Amino Purine within six weeks of culture. The plantlets were hardened in a 2:1 potting mixture of sand and soil and were established under greenhouse conditions. Hence, in vitro propagation protocol of siegsbeckia orientalis has been standardized.

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