Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 11, Issue 3, 2009; Page No.(573-575 )

PRODUCTION AND ENZYME ACTIVITY OF AN EXTRACELLULAR AMYLASE FROM ASPERGILLUS NIGER, ASPERGILLUS ORYZAE AND BACILLUS SUBTILIS

B.A. AGLAVE, P.P, LELE, M.O. LOKHANDE AND R.S. PAGARE

Abstract

The study of amylase production were carried out with Bacillus subtilis bacterial and Aspergillus mger and Aspergillus oryzae fungal organism. The Bacillus were cultured in a mineral medium containing yeast extract, peptone, MgSO4, NaC1, CaC12 and Aspergillus were cultured in a mineral medium incorporated with glucose, glycerol and yeast extract. The minerals in media shortened the lag phase greatly and improved the growth and amylase synthesis. The optimum temperature and pH for activity were 40°C and 7.0 respectively. The addition of substrate concentration at 1% exhibits maximum amylase activity.

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