Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 1 Issue 1-2, 2000; Page No.(51-55 )

MICROTOX RESPONSE TO RIVER WATER CONTAMINATED WITH AZODYES

P.B. DOCTOR,. S.K. GHOSH, A. DMASARI AND P.K. KULKARNI

Abstract

Dye-contaminated discharge from the cotton textile printing factories situated at the Jetpur town on the upstream of the river endangers the quality of the potable water supplied to Dhoraji.town, situated on the downstream of the same river. Samples of dye-contaminated water were collected from four up-stream sites of the river Bhadar near the Jetpur town and one from downstream, Dhoraji to determine existing or potential impacts of zao-dye associated toxicity of the water with the Microtoxlm system using Pholobacterium phosphroeum as the sensor organism. Samples of Site 1 caused a negative value of (7i, which indicated the samples were not only non-toxic but stimulated luminescence. Samples at Sites 2 and 3 were likely to be of less toxic as the value of effective concentration at 50% level (EC50) could not be evaluated due to less decrement of luminescene (<50%). But the light output decreased considerably (>50%) at Sites 4 and 5 of which Site 4 was the most toxic (EC5o 4.85% at T15°C t15min) compared to Site 5 (EC50 18.80% at t1°C t,5min). Influence of physical factors were studied on the determination of EC50 values of the contaminated river water. It was observed that time, temperature and colour adjustment had significant effect on the determination of EC50 at each Site (p <0.001).

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