DETECTION AND OPTIMIZATION OF INDOLE ACETIC ACID (IAA) PRODUCTION FROM RHIZOBILIM MELILOTIV.P. GHODAKE, S.A. PATIL, P.P. PATTEKARI, P.A. DHUMAL AND S.D. KHAMBE
In this study Rhizobium sp. was isolated from root nodules of fenugreek (Trigonella foenum-graecum) and Identified as Rhizobium meliloti following standard methods. This isolate was tested for the production of indole acetic acid (IAA). Detection of IAA was done with TLC where a specific spot from the extracted IAA preparation was found, corresponding with the standard spot of IAA with same Rf value. The addition of L. tryptophan (as a precursor for auxins) to the medium inoculated with Rhizobium meliloti, enhanced the production of IAA several fold compared to L-tryptophan non-amended medium. Moreover, the present results support the consensus that the tryptophan dependent pathway plays vital role to maintain very high IAA levels. The temperature 30°C, pH 7.0 and 0.4 % tryptophan concentration in, the medium was found optimum for the production of IAA by Rhizobium meliloti. Production of IAA by Rinzobium meliloti increased with an increase in tryptophan concentration from 1 to 4- mg/mL.
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