Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 10, Issue 1, 2008; Page No.(91-96)

PRODUCTION OF MICROBIAL INOCULANTS: COMPARISON OF LIQUID AND CHARCOAL BASED BIOFERTILIZER TECHNOLOGY

RANJANA BHATIA, RAKESH KUMAR, MANJULA VASUDEVA, BALJEET SINGH SAHARAN, RAJ KUMAR BANSAL AND NEERU NARULA

Abstract

In this investigation we describe the use of different available carriers suitable for the use of bioinoculants (Azotobacter). Most widely used charcoal based technology was compared with liquid based technology under laboratory and field conditions on cotton crop. Under laboratory conditions, survival of Azotobacter in charcoal and liquid based conditions was studied at three different temperatures (30, 37 and 45°C) up to 7 months. Maximum count was obtained by A1a27 (6.06 x 108 cfu m1-1 at 30°C on 50 days, 5.06 x 108 cfu m1-1 at 37°C on 40 days), AVK51 (6.65 x 108 cfu m1-1 at 30°C on 50 days, 7.36 x 108 cfu m1-1 at 37°C on 40 days) as liquid based cultures. At 45°C, after 50 days, liquid based cultures HT54 (1.34 x 108 cfu ml-1) and HT57 (1.06 x 107 cfu ml-') were better than charcoal based cultures. Yield increased with liquid based bio-inoculants (403.71 kg ha-1 with HT54) as compared to charcoal based cultures (303.71 kg ha' with AVK51) under field conditions. Our. results reveal that charcoal based. technology can be successfully replaced by liquid based technology.

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