Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 08, Issue 2, 2006; Page No.(345-348)

STUDIES ON EFFECT OF CARBON SOURCES, NITROGEN SOURCES, PH AND SURFACTANT ON CL, CX AND FILTER PAPER ACTIVITIES OF CELLULASE ENZYME FROM ASPERGILLLIS FLAVLIS

DEBABRAT BAISHYA AND MANAB DEKA

Abstract

Cellulases are complex enzyme system comprising of endo-1,4-13-D glucanase, otherwise known as endocellulase, carboxy methyl cellulase (CMase) or Cx cellulase; Exo-1,4-13-D-glucosidase or 13-D-glucosicle or glucanhydrolase. The enzymatic saccharification of cellulose has been proposed as a means of producing glucose on an industrial scale. The present study was, therefore, under taken in order to examine the Cx, C, and filter paper activities of the enzyme cellulase produced by a local fungal strain identified as Aspergillus flavus isolated from decaying wood so as to assess the potentiality of cellulase produced by the fungal isolate for saccharification of various soluble carbon sources, nitrogen sources and also to see the effect of pH and surfactant (Tween-20) on the activities of the enzyme. The production of cellulases by Aspergillus flavus in Mandel and Sternberg medium (1976) has revealed that the activities of C1, Cx and filter paper increased up to the 10th day of cultivation. However, this fungus shown maximum activities of C,Investigation on the effect of nitrogen sources revealed that the addition of Peptone (0.57 g/l) to the medium as nitrogen sources showed the maximum enzyme production as detected by enzyme assays. It has been observed that a pH of 3.0 showed maximum production of enzyme while the addition of Tween- 20 at a level 0.15% (v/v) to the medium established the influence of surfactant on the production of cell ulase.

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