CYTOTOXICITY TEST OF TWO DEAZAELLIPTICINE COMPOUNDS AGAINST LIVER CANCER CELLS (HEPG2 CELL LINE)Awik Puji Dyah Nurhayati, Mardi Santoso, Edwin Setiawan1, Syawlia, Rizki M., Fitri Lianingsih, Adik Mayang Indiani and Shabrina Syifa Ghaissani
Cancer is a genetic disease caused by DNA mutations. Doxorubicin, is one of the liver cancer chemotherapy agents. However, its use may provide cardiotoxic effects, myelosuppression and mucositis in normal cells. Therefore, the alternative of doxorubicin substances is need, one of the potential substances is ellipticine. This study was conducted to test the effect of cytotoxicity of two ellipticine compounds on HepG2 cell liver cancer line. The cytotoxicity test of the compound was carried out by using the MTT test method (3- (4,5- dimethyltiazole-2-yl) -2,5-diphenyltetrazolium bromide). Cell cultures were treated with compounds of 7 variations in concentrations (200, 100, 50, 25, 12.5, 6.25, 3.125). As a control, the treatment of doxorubicin is used. Optical density is measured by microplate reader. The results were analyzed using polynomial regression and displayed as IC50 values. Results of IC50 cytotoxicity of both compounds and doxorubicin were 1,932 Ã¬M; 2,990 Ã¬M; 0.642 Ã¬M.