IN VITRO TEST OF DEAZAELLIPTISINA 1 COMPOUND ON PHAGOCYTOTIC ACTIVITY OF MACROPHAGE CELLAwik Puji Dyah Nurhayati, Mardi Santoso, Edwin Setiawan, Ayuri Cintyasari and Syamsudin Abdillah, Fitri Lianingsih, Adik Mayang Indiani and Shabrina Syifa Ghaissani
Cancer is a growth of abnormal cells that invades tissues and possess a metastasis phase. Chemotherapy treatment is often used but it causes side effects, namely immunosuppressive nevertheless. Therefore, natural ingredients that function as immunomodulator is needed for improving immune system. Deazaellipticine 1 compound were synthesized from Ochrosia elliptica is a natural ingredient that possess an anticancer potent and being expected as an immunostimulant. This study was conducted to investigate in vitro test of Deazaellipticine 1 compound on phagocytosis activity of mice macrophage cells (Mus musculus). The concentration of Deazaellipticine 1 used is 6.25; 12.5; 25; 50; 100 ïg/ ml and as a positive control, an anticancer drug Doxorubicin was used. Phagocytic activity was expressed in a capacity and an index. The results showed was phagocytosis capacity by 64.4% and phagocytic index 2.29 were increasing at a concentration of 100 Î¼g/ml, while Doxorubicin yield only phagocytic capacity of 17.7% and a phagocytic index of 0.06 at a concentration of 25 Ã¬g / ml. Deazaellipticine 1 compound is able to increase the phagocytic activity of macrophage cells in mice (M. musculus).