Ecology, Environment and Conservation Paper

Vol 27, Feb Suppl. Issue, 2021; Page No.(48-57)

COMPARATIVE OPTIMIZATION STUDIES (ISP 4 VS ISP 3 VS ISP 2 MEDIA) OF MANGROVIAN STREPTOMYCES PLURIPOTENS ANUKCJV1 FOR ITS α - AMYLASE PRODUCTION AND GEOGRAPHICAL CORRELATION OF MANGROVIAN ACTINOMYCETES STRAINS

SK. Chand Basha, K.R.S. Sambasiva Rao and E. Venkatanagaraju

Abstract

Streptomyces pluripotens ANUKCJV1 was isolated from Coringa Mangroves which was located along the South Indian Delta. The Current work which was in continuation to our previously reported work which suggests that Streptomyces pluripotens ANUKCJV1 was the potential strain and the same has been subjected to comparative optimization studies in the current work by employing three media: ISP 4; ISP 3; ISP 2 media for enhanced α- Amylase Production. Physico - Chemical variables viz… Incubation period, pH, Temperature, Carbon and Nitrogen sources with respect to three different media (ISP 4, ISP 3 and ISP 2) were tested and cumulative analysis of three different media for differential bioactivity of α - Amylase was done. Results suggest that ISP 4 found to be the best medium with cumulative value of 24.2 U/mL, where as the cumulative value of ISP 3 and ISP 2 were 19.3 U/mL and 19.4 U/mL respectively. Peptone as Nitrogen source of ISP 4 found to be the favourite Individual variable among all with production value of 8.0 U/mL. Geographical correlation with respect to number of Actinomycetes strains and α - Amylase Bioactivity depicts that Distant geographical soil samples from the shore found to be favourable for higher number of Actinomycetes strains: A1 soil samples (~ 500 m) - 33 %; A2 samples (~ 400 m) - 22 %. With regard to α - Amylase Bioactivity, A5 samples (~ 100 m) analysed to be the potential geographical bioactive zone for α - Amylase Production. From the study it can be concluded that since ISP 4 found to be the favourite medium of the potential strain, by employing the same large scale exploration of the Streptomyces pluripotens ANUKCJV1 of the Coringa Mangroves may be done to tap the industrial benefits of α - Amylase.

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