Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol.14, Issue 1, 2012; Page No.(69-74)

ISOLATION AND CHARACTERIZATION OF MICROBES FOR PRODUCTION OF ENZYME

R. K. Pathak, G. Glen and Nitin E. Pereira

Abstract

Protease are among the oldest enzyme known to human being with the molecular weight ranging from 18 to 90 Kda. They are the enzymes that catalyzes the splitting of proteins into smaller peptide fractions and amino acids by a process known as proteolysis. These are found in wide diversity of sources like plant, animals and microorganisms. The microorganisms being the major source. Protease catalyzes the total hydrolysis of protein and have broad scope of usage in the industries. The increasing demand of protease in industries led to isolation of microorganisms producing protease. In the present study the microorganisms were isolated from alkaline soil collected from beach. The serial dilution was performed and 5 bacterial strains were isolated. The growth characteristics were studied. The microorganisms producing protease were screened using folin- lowry method and the activity of enzyme was determined.

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