ISOLATION OF A MESOPHILIC STRAIN OF AS PER GILLUS WENTII CAPABLE OF DECOLONIZING TEXTILE DYEING MILL EFFLUENTS.C. KALE AND A.J. GAVHANE
In all nine fungal isolates were obtained from various sources such as soil, deteriorated paints and decaying wood collected from Karad (Maharashtra, India) locality, by enrichment culture technique followed by isolation by dilution plate technique, using Sabouraud Glucose Agar and incubating the plates at R. T. All nine isolates were subjected to primary screening by spot inoculation â Agar plate method using modified Sabouraud Glucose Agar (with various concentrations of textile effluent). Primary screening yielded four isolates designated as F - II, F - III, F - IV and D-1 capable of decolonizing the waste at 20% conc., V/V. These four isolates were subjected to secondary screening by liquid culture method. Secondary screening revealed that fungal isolate F-III was the best among st the isolates as it showed maximum decolonization (91.38%) of 1:5 diluted effluents at 37 oC within eight days. The fungal isolate being of interest was then studied for its morphological and cultural characteristics and was identified as a strain of Aspergillus wentii Wehmer. The identity of the culture was confirmed by referring to the Fungus Identification Service Center.