IN VITRO PROPAGATION OF TINOSPORA CORDIFOLIA AND ESTIMATION OF BERBERINE CONTENT BY CHROMATOGRAPHIC ANALYSISSukhvinder Singh Sudan, Bhavika Batra, Tushar Gusain1, Ankita Lal and Manu Pant
The present study was designed to establish a propagation method of Tinospora cordifolia using tissue culture technique. Nodal explants of field grown mature plants of T. cordifolia were used to initiate the cultures. Conditions were standardized to establish contamination free shoot cultures on nutrient medium fortified with plant growth regulators. In vitro raised cultures and shoots from mother plant were assessed for presence of the secondary metabolite. Thin layer chromatographic analysis was done and presence of berberine in shoot cultures was established.