Pollution Research Paper

Vol 38, Issue 4, 2019; Page No.(959-964)

ISOLATION AND MOLECULAR DIFFERENTIATION OF MDR BACTERIA ISOLATED FROM DAIRY PRODUCTS

WALEED K. ALKHAFAJE, SAFAA M. ALI AND ZAKIA A. OLAMA

Abstract

Antibiotics are extensively used in human, veterinary medicine and in agricultural settings for the treatment of infectious diseases, growth enhancement and prophylaxis in food animals, potentially leading to selection of drug and multidrug resistant bacteria. The prevalence of the MDR bacteria in dairy product was evaluated. Thirty (30) out of 131 bacterial isolates were proved to be MDR. All the selected bacterial isolates (30) were resistant to metronidazole (MTZ). In general, the interaction to the additional used antibiotics (19) was varied. The isolates and selected MDR bacterial isolate from different samples were distributed as follows: 15 from kareish cheese, 11 industrial white cheese, 4 old cheese and 2 romy cheese. RAPD PCR was used to confirm the molecular variation between all the selected bacterial isolates (30) by using 4 different prime which show the variation between the selected bacterial isolates. Phenotypic and biochemical identification of all the selected isolates (30) revealed that (E.coli, Staphylococcus sp, Psedoumonas sp, Enterococcus sp, Shigella sp, Bacillus sp and Proteous sp). 13 isolates out of 30MDR strains were Staphylococcus sp. (43.33%). Molecular studies of plasmid profile revealed that not all the selected bacterial isolates have plasmids with size less than 5 kb.

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