Ecology, Environment and Conservation Paper

Vol 25, Nov Suppl. Issue, 2019; Page No.(187-193)

ROLE OF WHITE ROT FUNGUS (TRAMETES HIRSUTA MTCC 136) IN THE BIOAUGMENTATION OF NATURAL ORGANIC MATTER WASTES FROM WATER RESERVOIRS

Jeenathunisa, Jeyabharathi S. and Stephan R.

Abstract

Indeed, it is the need of the hour to provide the pure water from natural reservoirs for the public domain to satisfy their water requirement for domestic, commercial and industrial purposes. During large water reservation in dams and other artificial tanks, the undesirable formation of NOM (natural organic matter) wastes consists of non-humic and humic substances were considerably high. The consistent accumulation of NOM affects the taste and odour of the water that are considered as the organic pollutant which disturbs the aquatic ecosystem. Hence, the present study facilitates the safe removal of NOM as was suggested by fractionating the sample with DAX 8, XAD 4 and IRA 958 synthetic resins to obtain very hydrophobic acids (VHA), slightly hydrophobic acids (SHA), hydrophilic charged (CHA) and hydrophilic neutral (NEU) compounds. Then the molecular weight of both whole NOM fractions was measured using Highperformance size-exclusion chromatograph (HPSEC). The results were compared before and after inoculation with the Basidiomycetes family fungi Trametes hirsuta MTCC 136 showed a significant reduction in molecular weight of samples treated with Basidiomycetes family fungi Trametes hirsuta MTCC 136 by the production of phenoloxidease enzyme Laccase which was confirmed by Guiacol test.

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