UPTAKE OF METAL IONS FROM ARTIFICIAL GREY WATER BY BOTRYOCOCCUS SP.RADIN MAYA SAPHIRA RADIN MOHAMED, MUHAMMAD SAFWAN MISWAN,ADEL ALI SAEED AL-GHEETHI, AMIR HASHIM MOHD KASSIM,WUROCHEKKE ANWARUDDIN AHMED1 AND NURINA FITRIANI
Grey water from bath roomsare directly discharging into the drain or stream without any treatment in the rural area, due to the absence of a central treatment plant. These practices become unacceptable because its lead to water pollution and aggravate mosquito nuisance. The study aimed to investigate the potential of Botryococcus sp. cell to uptake of metal ions (Ca, Na, Mg, Feand Zn) from artificial grey water samples during the phycoremediation process. Botryococcussp. was inoculated into the phycoremediation flasks with 8 log10 cell mL-1 and incubated at room temperature for 3, 5, and 7 days. The highest uptake was 99.7% for Ca, 83% for Na, 96% for Mg, 64%for Fe and 98.8% for Zn ions. The specific uptake rate of Ca, Na, Mg, Fe and Zn ions have a significant relationship with initial concentration in the artificial grey water (R2= 0.93, 0.61, 0.92, 0.76 and 0.86 respectively). The kinetic coefficient of grey water parameters uptake by Botryococcussp. was determined as k=2.58 mg Ca 1 log 10 cell mL-1 d-1 and km=4.43 mg L-1 (R2=0.89), k=0.93 mg Na 1log 10 cell mL-1 d-1 and km=22.57 mg L-1 (R2=0.79), k=0.97 mg Mg 1 log10 cell mL-1 d-1 and km=1.56 mgL-1 (R2=0.98). k=0.39 mg Fe 1 log10 cell mL-1 d-1 and km=14.39 mg L-1 (R2=0.63) and k=0.21 mg Zn 1 log10cell mL-1 d-1 and km=1.3 mg L-1 (R2=0.77). Yield coefficient for Ca, Na, Mg, Fe and Zn ions removalby specific growth rate of Botryococcus sp. were 6.01, 10.35, 4.5, 0.57 and 0.54 respectively.
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