Ecology, Environment and Conservation Paper

EFFECT OF NITRIC ACID MUTATION OF BACILLUS SUBTILIS ON SEEDS GERMINATION OF VIGNA RADIATA

Zh. B. Suleimenova, O.N. Shemshura, A.K. Sadanov, G.A. Mombekova and Zh. K. Rakhmetova

Abstract

Plant growth promoting rhizobacteria (PGPR) are beneficial bacteria that colonize the roots of plants and accelerate plants’ growth. Strain Bacillus subtilis isolated from the rhizosphere of mung bean (Vigna radiata) in the South Kazakhstan, was used in this research. Bacillus subtilis strain was allowed for chemical mutagen (nitric acid) to cause mutation, and observed for its consequent action on the growth of Vigna radiata. The ability of the parent and mutant strains to synthesize indole acetic acid (IAA) has been studied. It has been found that on the 3d day of cultivation, the IAA concentration in the mutant strain was significantly higher than in the parent strain. The energy of germination of mung bean seeds was determined on the 4th day after seeds treatment with culture broth. The mutant strain Basillus subtilis M-2 was found effective to enhance the germination energy of mung bean seeds compared to parent strain Bacillus subtilis. Treatment of the mung bean seeds with mutant strain of Bacillus subtilis M-2 had a significant stimulation effect on plant growth. The overall germination rate of the mung seeds bean treated with mutant strain increased by 17 %, compared to parent strain. The length of the stems and roots of mung bean treated with Bacillus subtilis M-2 increased significantly in comparison with parent strain in 1.8 and 1.5 times, respectively.