Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 21, Issue 1, 2019; Page No.(46-52)

CHARACTERIZATION OF ANTIMICROBIAL ACTIVITY OF GOLD NANOPARTICLES PREPARED BY PULSE LASER ABLATION IN LIQUID

ABEER A. ABASS, WASNA’A M. ABDULRIDHA AND ZUHAIR S. ALSEHLAWI

Abstract

The main objective of this Research was to prepare and evaluate the antimicrobial activity of AuNPs nanoparticles against the antibiotics resistant bacteria included Escherichia coli, Pseudomonas aeruginosa and Proteus sp. Gold nanoparticles (AuNPs) was prepared by pulsed laser ablation in liquid (PLAL). The synthesis of nanoparticle were characterized by different techniques such as the diffraction of X-ray meam (X-RD), transmission electron microgram (TEM), atomic force microscopy (AFM), and absorption spectra of AuNPs (UV-Vis) spectra. The XRD displays Bragg’s diffraction peaks located at (110), (200) and (220) facts in the face-centered polycrystalline structure. Size and shape of nanoparticles were determined by TEM microgram with size ranging 50±5nm forming flower-shapes. Escherichia coli, Pseudomonas aeruginosa and Proteus sp. were isolated and identified from different clinical samples using traditional methods on bacteriological culture media. Antibacterial activity of AuNPs against multi-drug resistant bacteria were assayed qualitatively and quantitatively using two method; well diffusion and micro dilution, respectively. The antibacterial susceptibility of (Amoxicillin, Cefotaxime, Gentamicin and Ciproflouxacin) were analyzed on Muller Hinton agar (MHA) plate. The results of antibacterial susceptibility of bacterial isolates exhibited high resistance to the antibiotics that used in the current study. According to these findings gold nanoparticles revealed highest activity when using 100 μg/mL in well diffusion and the mean of inhibition zone was 26-37 mm for each Escherichia coli, Pseudomonas aeruginosa and Proteus sp. However, the minimum inhibitory concentration (MIC) values of gold nanoparticles recorded 75 and 73 μg/mL against Proteus sp. and P. aeruginosa, respectively. And 53 μg/mL was a lowest concentration of AuNPs for inhibiting E. coli. It is concluded that the AuNPs nanoparticles have in vitro effectiveness and appeared to be a good antibacterial agent against the multidrug resistant bacteria.

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