A STUDY ON THE PHYSIOLOGICAL, BIOCHEMICAL AND LIPID CONTENT BEHAVIOUR OF MICROALGA CHLORELLA SP. IN RESPONSE TO IRON (III)YOGESH NEGI, JONETO NONGMAITHEM, FRANKIE J. LALOO AND SAMRAT ADHIKARI
Microalgae are potent organisms for biofuel production and their productive ability can be further enhanced when exposed to various environmental stress conditions. To identify the appropriate conditions suitable for high biomass and lipid productivity, Chlorella sp. was isolated from iron leaching areas and cultivated under various Fe3+ concentrations at pH 6.8. Highest growth and biomass productivity was reported for the group supplemented with 12μM FeCl3.Further, with the optimal iron concentration identified, the cells were subjected to both Fe3+ (12μM) and pH stress (5 and 8). The results showed that the total lipid content for Chlorella sp. cultured with 12 μM FeCl3 at pH 8.0 and those grown at pH 5.0 stress achieved 52.8% and 38.8% biomass by dry weight and was 7.3 and 5.3 times respectively higher than the control group at pH 6.8. The physicochemical abilities of these cells at 12 μM Fe3+ was also analyzed to assess the effect of iron on these microbial cells.
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