Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 20, Feb. Suppl. Issue, 2018; Page No.(99-103)

UTILIZATION OF MOLASSES IN PRODUCTION OF POLY-β- HIDROKSI BUTIRAT (PHB) BY ISOLATE OF BACTERIA FROM WASTE SUGAR MILL IN BONE, SOUTH SULAWESI

NUR HAEDAR, AS’ADI ABDULLAH, HAMSINAH AND MUSTIKA TUWO

Abstract

The aim of this study was to understand the ability of the isolates of bacteria from Sugar Mill in Bone, Soth Sulawesi, specifically KC1, BA9, and MA4, and also the optimal concentration of molasses to produce PHB.Bacteria were isolated using the medium Nutrient Agar. The concentration of molasses in this research were 1%, 2%, and 3% and the positive control was glucose 1%. The quantitative test was conducted by counting the total dry weight of cell mass. The analysis of the amount of PHB used spectrophotometer UV-VIS with wavelength 235 nm. The result showed that isolates of KC1 was the most effective since it produced PHB with a quantity of 5,841 % per dry weight of the cell mass, while the isolates of BA9 produced 3,736 % PHB per dry weight of the cell mass, and the isolates of MA4 made PHB with a quantity of 3,635 % per dry weight of the cell mass. The molasses with 2% of concentration was optimal to accumulate PHB by the isolates of KC1 with 72 hours of incubation, while the isolates of BA9 and MA4 were optimal in the molasses with 3% of concentration and 72 hours of incubation. KC1 was the most effective isolate to produce PHB compared to BA9 and MA4 isolates and optimally accumulate PHB at a molasses concentration of 2%.

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